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</html>";s:4:"text";s:19730:"This real-time PCR-based kit is designed for the quantification of amplifiable human DNA that is present in a sample, and has been validated for human identification applications. Special emphasis was put on PCR efficiency, detection limit and detection range. English. FAQ | Download our Company Profile; Principals. The other assays listed above were not available at that time. Forensic DNA Analysis . human DNA in a sample. 4387746 Publication Number 4391294 Revision E For Research, Forensic, or Paternity Use Only. Barbisin et al. The Quantifiler Duo kit from Applied Biosystems simultaneously provides quantitative and qualitative assessment of total human and human male DNA, enabling forensic laboratories to obtain more . given the growing number of casework samples submitted by law enforcement, forensic laboratories constantly seek new ways to improve efficiency, the quantifiler duo kit is a powerful new tool which provides more comprehensive information in a single quantification reaction, and it has been optimized and validated as part of a complete dna results obtained with the quantifilerduo kit provide an indication of the presence of inhibitors, allowing analysts to re-purify samples prior to The . In this paper, the feasibility of using the Quantifiler() Trio DNA quantification kit for the direct quantification of forensic casework samples was investigate The effectiveness of a direct quantification assay is essential to the adoption of the combined direct quantification/direct STR workflow. DNA Quantification Solutions. Quantifiler Duo Product Comparison Quantifiler Duo DNA Quantification Kit is designed to simultaneously quantify the total amount of amplifiable human DNA and human male DNA in one reaction. Identifiler Plus (Forensic DNA Identification Kits) Rated 0 out of 5. 4485354, Rev B. Applied Biosystems (Foster City, CA) has recently developed a new DNA quantification kit, Quantifiler Duo, which simultaneously quantitates the total amount of amplifiable human and male DNA present in a sample. The kit can reproducibly detect quantities as low as 5.75 pg/L for the human assay (limit of detection), with some variation for the SRY specific assay due to its haploid nature [20]. 2 Follow the protocols that are described in the Quantifiler Duo DNA Quantification Kit User Guide and run the samples on an Applied Biosystems 7500 Real-Time PCR System Limited product warranty Validation studies were designed to evaluate the sensitivity of the Quantifiler Trio kit and its performance with mixture and degraded samples. 78KB. Forensic DNA Analysis . DNA Quantification Solutions . An internal PCR control is formulated into each reaction to identify samples that may contain PCR inhibitors. The Quantifiler Duo kit has been optimized and validated as part of a complete DNA testing system with the AmpFLSTR PCR Amplification Kits. Not for diagnostic procedures. no. Quantifiler Duo Product Information human DNA in a sample. The kit can. Quantifiler Y Human Male DNA Quantification Kit (Life Technologies) SRM 2372 was characterized with the published Alu and CFS assays and the commercial Quantifiler Human and Quantifiler Y Human Male assays in 2006. DNA Quantification Kits QuantStudio 5 Real-Time-PCR Instruments for HID Real-Time PCR Analysis Software for HID The Quantifiler Duo DNA Quantification Kit enables forensic laboratories to simultaneously obtain a quantitative and qualitative assessment of total human and human male DNA in a single, highly sensitive real-time PCR reaction. Note: These detectors allow you to import the converted qPCR/STR system-generated 7500 setup file to the SDS Software. Abstract Abstract: The Quantifiler Duo DNA Quantification kit enables simultaneous quantification of human DNA and human male DNA as well as detection of inhibitors of PCR in a single real-time PCR well. The maximum yield of DNA from cotton fabric 12 hours post-deposition was 7 pg/ul and 5 pg/ul for total human . Q Protocol description for use with the Quantifiler Duo Quantification Kit. Forensic DNA Analysis . For use with: QuantifilerHuman DNA Quantification Kit (Cat. Abstract: The Quantifiler Duo DNA Quantification kit enables simultaneous quantification of human DNA and human male DNA as well as detection of inhibitors of PCR in a single realtime PCR well. 12 Quantifiler HP and Trio DNA Quantification Kits User Guide Chapter 1 Overview 1 Chemistry overview improved over Quantifiler Duo, Human, and Y Human Male DNA Quantification Kit assays. Information in this document is subject to change without notice. Pooled human male genomic DNA is used to generate standard curves for both human (ribonuclease P RNA component H1) and human male (sex determining region Y) specific targets. The Quantifiler Duo DNA Quantification kit enables simultaneous quantification of human DNA and human male DNA as well as detection of inhibitors of PCR in a single real-time PCR well. DNA Quantification Solutions Quantifiler Duo Kit Components Quantifiler Duo Kit Components Each Quantifiler Duo kit includes all reagents necessary for the amplification, detection, and quantification of 400 samples in 25 ul reactions using the Applied Biosystems 7500 Real-Time PCR System. The primary quantification targets (Small Autosomal and Y) consist of relatively short amplicons (75 to 80 bases) to improve the detection of degraded . Highly Accurate Determines the ratio of human male to female DNA, even with minute male and excess female DNA (1:4000 or greater) Highly Sensitive <1 pg/L limit of detection. 2. Text ConventionsThis guide uses the following conventions: Bold indicates user action. The Quantifiler kit generated the lowest DNA concentrations. Quantifiler Duo DNA Quantification Kit. Illescas, Mara J., Richmond VA; Mercedes, Fernando, San Juan PR; Dawson Cruz, Tracey, Richmond VA; Tirado, Carmen A., San Juan PR The Quantifiler Human DNA Quantification Kit and the Quantifiler Y Human Male DNA Quantification Kit were designed for the quantification of human genomic DNA in forensic samples. The DNA quantity of the stock was previously determined with the Quantifiler Duo Quantification kit (Thermo Fisher Scientific). no. The multiplex assay can quantify total human DNA and human male DNA simultaneously, determine the male to female DNA ratio, and detect PCR inhibitors in a single TaqManassay reaction. Quantifiler Duo Kit Configuration As with our Quantifiler Duo, Human, and Y Human Male DNA Quantification Kits, these kits use TaqMan quantitative real-time PCR technology. This paper describes a multiplex TaqMan real-time PCR assay, the Quantifiler Duo DNA Quantification Kit, for simultaneous quantification of human nuclear and human male DNA, as well as detection of the presence of PCR inhibitors in a biological sample. The Quantifiler Trio kit and the HID v1.2 software are designed for use with the Applied Biosystems 7500 Real-Time PCR System. Letter re Introduction of Quantifiler DNA Quantification Kit Raw Material Changes 344797.1 1 180 Oyster Point Blvd. In fact, this kit is at least 10 times more sensitive than the currently used Quantifiler Duo kit and can detect a male minor contributor at a level four times lower. QuantifilerDuo DNA Quantification Kit User Guide7 About This Guide IMPORTANT! Quantifiler Duo Articles 4343895) QuantifilerY Human Male DNA Quantification Kit (Cat. Get Adobe Reader. The new quantification kit known as Quantifiler Trio is introduced as more robust and sensitive compared to the Quantifiler Duo, providing additional information in the analysis of forensic samples. Pub. The new DNA quantification kits use multiplex TaqMan() assay-based fluorescent probe technology to simultaneously quantify up to three human genomic targets, allowing samples to be assessed for total human DNA, male contributor (i.e., Y-chromosome) DNA, as well as a determination of DNA degradation state. DNA and detect the presence of PCR inhibitors in a sample at an early stage in the analysis process. TaqMan real-time PCR assays, like the Quantifiler Human DNA Quantification Kit and the Quantifiler Y Human Male DNA Quantification Kit, have proven useful in addressing this need while providing numerous advantages over Language. This enables it to function as a critical component of an improved and integrated workflow for more effective processing of forensic casework samples. Follow the protocols described in the Quantifiler Duo DNA Quantification Kit User's Manual and run the samples on an Applied Biosystems 7500 Real-Time PCR System. IMPORTANT! DNA Quantification Solutions FAQs Agilent; Anton Paar; Three quantification runs with triplets of each dilution were set up on the Biomek 4000, placing the samples on different plate positions for each run. The Quantifiler Trio Kit is designed to simultaneously quantify the total amount of amplifiable human DNA and human male DNA in a sample. Species Specificity The DNA from nonhuman biological species was either obtained commercially or purified in the laboratory. Part Number 4391294 Rev 04/2008 Barbisin, Maura, et al. DNA Quantification Solutions . See the complete profile on LinkedIn and discover Dr. Mohammed's connections and jobs at similar companies. This study investigates the connection between the results of DNA quantification with Quantifiler Human DNA Quantification Kit (AB) and DNA profiling. For more information visit thermofisher.com This prompted a set of experiments designed to determine the source of variability. DNA Quantification Solutions . Laila Hasap, Wilaiwan Chotigeat, Jintana Pradutkanchana, Uraporn Vongvatcharanon, Thitika Kitpipit, Phuvadol Thanakiatkrai, A novel, 4-h DNA extraction method for STR typing of casework bone samples, International Journal of Legal Medicine, 10.1007/s00414-019-02232-9, (2020). For some of these DNA samples, the sex of the donor animal was unknown. For some spe-cies, multiple donor animals were tested. South San Francisco, CA 95080 T: (650) 244-1000 www.lifetechnologies.com January 31, 2014 Dear Valued Customer, . DNA Quantification Solutions . The quantification assay combines three 5' nuclease assays, namely: target-specific human DNA assay, target-specific human male DNA assay, plus an internal PCR control (IPC). DNA quantification was performed using the Quantifiler Duo Quantification Kit (Applied Biosystems, Foster City, CA) as per the manufacturer's recommendations [10] using the 7500 Detection System . It requires familiarity with the 7500 Real-Time PCR System and 7500 SDS software. View Dr. Mohammed Albujja's profile on LinkedIn, the world's largest professional community. 92 male/female mixtures with minute amounts of male DNA were quantified with the Quantifiler Duo Human Quantification Kit using 40 and 45 cycle conditions. We would like to take this opportunity to provide the latest Purpose: To evaluate the use of Quantifiler Trio for quantification of male and human DNA, and . For discussion, depending on the specific DNA concentration, the samples were divided into four groups (0-5, 5-10, 10 . the quantifilerduo dna quantification kit is designed to streamline the workflow of the forensic casework analyst by providing information that can be used to guide downstream sample processing. A shift in the cycle . 12 Quantifiler HP and Trio DNA Quantification Kits User Guide Chapter 1 Overview 1 Chemistry overview improved over Quantifiler Duo, Human, and Y Human Male DNA Quantification Kit assays. Quantifiler HP and Trio DNA Quantification Kits Quick Reference Card . The Quantifiler Human DNA Quantification Kit and the Quantifiler Y Human Male DNA Quantification Kit were designed for the quantification of human genomic DNA in forensic samples. The Quantifiler Duo DNA quantification kit (Thermo Fisher Scientific) was used for the direct qPCR assay. Learn More & Register Quantifiler Duo Kit Components The new quantification kit known as Quantifiler Trio is introduced as more robust and sensitive compared to the Quantifiler Duo, providing additional information in the analysis of forensic samples. The series will cover a range of topics important to this community highlighting the most recent methods and best practices in areas such as forensic chemistry (covering spectroscopy, microscopy, and other techniques), toxicology, trace evidence, DNA analysis, and many more. Forensic DNA Analysis . No. Not for diagnostic procedures. 4343906) Publication Number 4344790 Revision G For Research, Forensic, or Paternity Use Only. GlobalFiler STR Kits (Forensic DNA Identification Kits) Delivering Quality To Every Single Customer. The accuracy and precision of the Quantifiler Kit methods was comparable or superior to that of other quantification methods, and could detect as little as 32 picograms of DNA using 2 microL of sample per assay. Before using this product, read and understand the information in the "Safety" appendix in this document. The Quantifiler Trio Kit is designed to simultaneously quantify the total amount of amplifiable human DNA and human male DNA in a sample. Forensic DNA Analysis . 40 artificial stain . DNA Quantification Solutions . The As with our Quantifiler Duo, Human, and Y Human Male DNA Quantification Kits, these kits use TaqMan quantitative real-time PCR technology. For this purpose the DNA concentration of 3.068 routine casework samples was determined and DNA profiling was carried out. The Quantifiler Duo DNA Quantification Kit can quantify DNA sample concentrations between 23 pg/L and 50 ng/L when using the reagents in the kit. The Quantifiler HP and Trio Kits use . DNA Quantification Solutions . Quantifiler Duo DNA Quantification Kit User's Manual. Quantifiler Duo DNA Quantification Kit (Forensic DNA Identification Kits) Rated 0 out of 5. Quantifiler Duo DNA Quantification Kit USER GUIDE For Research, Forensic, or Paternity Use Only. File size. Download. developed the Quantifiler Duo DNA Quantification Kit that enables quantification of total human DNA and human male DNA and detection of the presence of PCR inhibitors in a. Quantifiler Duo DNA Quantification Kit User's Manual for details on creating the detectors. The primary Most of the reactions uti-lized 5.0 ng of input DNA. QuantifilerDuo DNA Quantification Kit For use with: Catalog no. Quantifiler Duo DNA Quantification Kit. J Forensic Sci, March 2009, Vol. DNA Quantification Solutions . The Quantifiler Duo DNA Quantification Kit can quantify DNA sample concentrations between 23 pg/ L and 50 ng/ L when using the reagents in the kit. DNA Quantification Solutions . The Quantifiler Duo kit uses the same chemistry that is used on the Applied Biosystems real-time PCR system for the amplification, detection and quantification of DNA in a sample to guide STR chemistry selection as the next step in the analysis. Forensic DNA Analysis . Not for diagnostic procedures. AudienceThis manual is intended for scientists who use the QuantifilerDuo DNA Quantification Kit for the quantification of human DNA extracted from a variety of sample types. The QuantifilerDuo DNA quantification kit was designed to address all these needs and in particular for processing sexual assault cases. 54, No. Quantifiler FAQs Quantifiler Duo Kit Components The Quantifiler Duo DNA Quantification kit enables simultaneous quantification of human DNA and human male DNA as well as detection of inhibitors of PCR in a single real-time PCR well. Dr. Mohammed has 5 jobs listed on their profile. The three kits gave different figures for the DNA content of the samples: The Quantifiler Duo kit produced higher DNA concentrations than the Quantifiler , and the Plexor kit higher DNA concentrations than the Quantifiler Duo . A 25-L qPCR reaction mix (10.5 L of Quantifiler Duo Primer Mix, 12.5 L of Quantifiler Duo PCR reaction Mix and 2 L of de-ionized water) was then added to the well containing a blank or a sample punch. pdf. Forensic DNA Analysis . Forensic DNA Analysis . 8QuantifilerDuo DNA Quantification Kit User Guide About This Guide Revision history 1 QuantifilerDuo DNA Quantification Kit User Guide9 Overview A review of 14 standard curves, using two different QuantDuo standard DNA lots, showed variability of cycle threshold values between assays were larger than those of the Internal PCR Control (IPC). The Quantifiler Duo DNA Quantification Kit is a real-time polymerase chain reaction (rtPCR) assay designed to use a small portion of an extract to estimate the total quantity of amplifiable human DNA and male DNA present in the sample. Format. Using Quantifiler Duo with the Yfiler Kit Online-only access $20.00 Details PDF download and online access $49.00 Details Check out Abstract Abstract: The Quantifiler Duo DNA Quantification kit enables simultaneous quantification of human DNA and human male DNA as well as detection of inhibitors of PCR in a single real-time PCR well. The new kit includes the same primers and allelic ladder as the widely used Identifil-er Kit and harnesses next-generation PCR amplification technology to help provide enhanced sensitivity, a cleaner baseline, improved performance on mixtures, and the ability to overcome high levels of PCR inhibition. The kit is a multiplex real-time PCR system based on the 5' nuclease assay and Taqman probe-based technology. Quantifiler HP and Trio DNA Quantification Kits User Guide. In this study the effect of increased cycle number conditions for Quantifiler Duo DNA Quantification Kit (AB) on the detection of minute amounts of male DNA in male/female mixtures was tested . DNA Quantification Solutions . The Quantifiler Trio DNA Quantification Kit is a robust kit that enables forensic laboratories to simultaneously obtain a quantitative and qualitative assessment of total human and human male DNA in a single, highly sensitive real-time PCR reaction. Furthermore, we tested the influence of the calibrator DNA included in the different kits on the absolute values. This guides selection of the optimal STR chemistry (autosomal, Y-STR or miniSTR) and streamlines the workflow while . In this study, we used two real time PCR platforms (Roche LightCycler 480 System and the ABI 7900 real time PCR instrument) to compare three commercial kits for DNA quantification. Because the kit is . Validation of the Quantifiler Trio DNA quantification kit. Quantifiler Trio DNA Quantification Kit 400 reactions 4482910 HID 7500 Calibration Starter Kit NA A25019 HID Real-Time PCR Analysis Software v1.2 Single user license A24664 References 1. Validation studies were designed to evaluate the sensitivity of the Quantifiler Trio kit and its performance with mixture and degraded samples. Forensic DNA Analysis . Abstract Forensic DNA Analysis . In this study the effect of increased cycle number conditions for Quantifiler Duo DNA Quantification Kit (AB) on the detection of minute amounts of male DNA in male/female mixtures was tested. The Quantifiler Duo DNA Quantification Kit enables forensic laboratories to simultaneously obtain a quantitative and qualitative assessment of total human and human male DNA in a single, highly sensitive real-time PCR reaction. Quantifiler FAQs Developmental Validation of the Quantifiler Duo DNA Quantification Kit for Simultaneous Quantification of Total Human and Human Male DNA and Detection of PCR Inhibitors in Biological Samples. Quantifiler Duo DNA Quantification Kit (Life Technologies) . The extracts were then quantified using the QuantifilerDuo DNA quantification kit. The detectors must have the exact names and capitalization shown below. The results obtained can aid in determining the quantity of extract needed for an STR . ";s:7:"keyword";s:38:"quantifiler duo dna quantification kit";s:5:"links";s:988:"<a href="https://api.o91.coding.al/pxzjxi/m12-fuel-impact-vs-screwdriver">M12 Fuel Impact Vs Screwdriver</a>,
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