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</html>";s:4:"text";s:37120:"Presumptive test . Examine for ⦠acidic pH < 4.5. Negative (-ve) Catalase. It seems a reasonable mistake). Expected Results The catalase enzyme breaks down hydrogen peroxide in to water and oxygen The oxygen causes bubbles to form within seconds , indicating a positive test. <a href="https://www.uwyo.edu/virtual_edge/results/emb_results.htm">EMB Plate Results: - University of Wyoming</a> To aid in the differentiation of Enterobacter aerogenes and Escherichia coli. During the first period, inoculations of four kinds of media will be made for these tests. Pseudomonas aeruginosa is a glucose nonfermenter and is thus MR- and VP-. The results showed that the degradation of oil and grease was possible by the application of extracted bacterial isolates. It contains two dyes in the ratio of 6:1 and is both toxic to gram-positive microorganisms. Ideal test to identify between Escherichia coli (E.coli) and Enterobacter aerogenes (EA). TOP. Triple sugar iron agar (TSI) is a differential medium that contains lactose, sucrose, a small amount of glucose (dextrose), ferrous sulfate, and the pH indicator phenol red. ... Two common organisms that cause ropiness are Alcaligenes viscolactis (viscous) and Enterobacter aerogenes. It can be found in dairy products, soil, and the gastrointestinal tract of animals. Inoculate the organism directly onto the surface of an EMB agar plate and streak for isolation. Thereâs a big long list of similar looking tubes and agar plates to inoculate! 3. Enterobacter were proposed as a genus in 1960 by Hormaeche and Edwards based on the division of the former genus Aerobacter into motile, ornithine decarboxylaseâpositive strains (Enterobacter) and nonmotile ODC-negative strains (Klebsiella).The type species of the genus is Enterobacter cloacae.Enterobacter are ubiquitous and can be isolated from natural ⦠A/AG) Escherichia coli Bacillus subtilis Alcaligenes faecalis Enterobacter aerogenes Proteus vulgaris Pseudomonas fluorescens Bacterial unknown KIA Phenol red (pH indicator) below pH 6.8 above pH 8.2 6.8 â 8.2 Should ⦠microbiology unknown enterobacter aerogenes. Enterobacter aerogenes is positive. 1 / 72 Basic Characteristics. Inoculate 1/3 culture of test organisms Escherichia coli, Enterobacter aerogenes and Klebsiella pneumoniae into empty tubes and keep them aside for this test (Voges-Proskauer test). This raises the pH , turns the pH indicator to a blue color and represents a positive citrate test. microbiology unknown enterobacter aerogenes. Description: Enterobacter is a genus of Gram-negative bacteria which belongs to family Enterobacteriaceae.motile by peritrichous flagella facultatively anaerobic.Enterobacter are ubiquitous in nature and can be found in soil,water,sewage and intestinal tracts of animals.Enterobacters are opportunistic and nosocomial pathogens including species such as ⦠The purpose of this test was to determine if the bacterium could ferment mannitol (McDonald et al., 2011). After incubation, the four litmus milk tube cultures were observed for color change and curd formation at the base of the tube. Litmus milk test- Acid orgs. It is both a selective and differential mediums for coliforms. Enterobacterial Infections: Definition Enterobacterial infections are disorders of the digestive tract and other organ systems produced by a group of gram-negative, rod-shaped bacteria called Enterobacteriaceae. Like the Maltose, this test utilized the same procedures, loop inoculating and allowing for an incubation period. E. coli and Enterobacter aerogenes. Image Source: MicrobeWiki and Getty Images. 4. (2) Litmus Milk. By Prof Jeremiah Seni. When an organic acid such as citrate (remember Krebs cycle)is used as a carbon and energy source, References. The positive test indicated that the unknown, E. aerogenes does not produce acetyl methyl carbinol. Hari was tasked with seducing the Crown Prince of Germany, who had recently been placed in command of an army. Research shows that two clinical strains of E. aerogenes exhibited phenotypes of multiresistance to β-lactam antibiotics, fluoroquinolones, chloramphenicol, tetracycline, and kanamycin. Both strains showed a porin pattern different from that of a susceptible strain. It is used to differentiate enterics based on the ability to reduce sulfur and ferment carbohydrates. Last updated: August 15, 2019 by Sagar Aryal. Lastly casein test was performed, test showed positive result for the break down of casein. What does DNase test for and what are the results? Incubate the test controls at 37° C for phenylalanine deaminization, and litmus milk reac- 24â48 hours. Download scientific diagram | Colonies of Enterobacter aerogenes on blood agar. The gram stain showed red colored rods. 100% (1 rating) Enterobacter aerogenes and Klebsiella pneumoniae, both are Gram-negative and rod-shaped bacteria. Observe for growth, color change and gas reactions. Emerson Ronald K. Bernardino RMT,RN,MAN Diagnostic Test for Gram negative bacilli (Enterobacteriaceae) 1. In the litmus milk test, litmus acts as the acceptor of the hydrogen ion. unknown lab essay â free papers and essays examples. CAMP factor is a diffusible, heat-stable protein produced by group B streptococci. Litmus Milk Test Klebsiella Pneumoniae Fullexams Com. Results and Discussion All cultures employed in the study were pure. Among the Enterobacter aerogenes (i.e., Aerobacter aerogenes) species, Enterobacter cloacae, Enterobacter sakazakii, and Enterobacter hormaechei have caused sepsis and a severe form of necrotizing meningitis in neonates [129â134]. Thus, the alkaline-slantâacid-butt reaction on KIA is an important indicator that the test organism is a nonâlactose-fermenter. A/AG) Escherichia coli Bacillus subtilis Alcaligenes faecalis Enterobacter aerogenes Proteus vulgaris Pseudomonas fluorescens Bacterial unknown KIA Phenol red (pH indicator) below pH 6.8 above pH 8.2 6.8 â 8.2 Should ⦠After incubation, the four litmus milk tube cultures were observed for color change and curd formation at the base of the tube. Litmus milk This medium has 10% powdered milk and a small amount of litmus as a pH indicator (pH starts at 6.8). Observation and Results Description of Unknown's Characteristics Abundant, thick, white, glistening growth Organism: Enterobacter aerogenes Experimental Procedure Observations Results Litmus Milk Reaction Pink(Acid) Carbohydrate Fermentations: A/G Lactose Dextrose A/G Sucrose A/G Hydrogen Sulfide Production No black precipitate formed Nitrate Reduction red ⦠Then it was confirmed by the instructor that it was the right gram negative found. P. aeruginosa. microbiology unknown by abbey marsh on prezi. Triple Sugar Iron Agar. Microbiology Unknown Enterobacter aerogenes. ⦠Gram-negative means that the organisms do not retain the violet color of the dye used to make Gram stains. He also used a preliminary enrichment medium, consisting of broth or peptone water and bile, after which he plated on solid mediums. In the hospital, the principal reservoir of K. pneumoniae is the gastrointestinal tract of patients. This test consisted of stirring the bacterial growth into a tube of phenol red and urea to test for the presence of acid. After incubation the broth was still a yellow color, giving a negative result. This confirmed that Enterobacter aerogenes was the gram-negative bacterium. (1) Carbohydrate (Sugar) Fermentation. I tend to listen to Martha Beck's books over and over. E. coli - note metallic sheen = lactose fermentor . Identification of an Unknown Bacterium and Writing Up a Report. Litmus Milk Test Klebsiella Pneumoniae fullexams com. Formerly known as the Bacterium aerogenes, Aerobacter aerogenes, and Cloacae,the name Enterobacter is now the accepted name of this genus, which is characterized by rod-shaped bacteria with, except for E. kobei, a positive Voges-Proskauer-test, motility, and ability to ⦠Observe daily for 7 days for alkaline reaction (litmus turns blue), acid reaction (litmus turns pink), indicator reduction, acid clot, rennet clot, and peptonization. Biochemical Test and Identification of Enterobacter cloacae. The lab instructor confirmed this was correct. Coliform bacteria are indicative of fecal contamination; therefore, a milk sample with a high coliform count is suspicious of harboring enteric pathogens also, such as Shigella or Salmonella. Inoculate tubes with the test specimen. 3. Pursuant to paragraph 74(b) of the Canadian Environmental Protection Act, 1999 (CEPA), the Minister of the Environment and the Minister of Health have conducted a screening assessment of Enterobacter aerogenes strain ATCC Footnote 1 13048.. E. aerogenes strain ATCC 13048 is a bacterium that has characteristics in common with other strains of the ⦠MICROBIOLOGY UNKNOWN LAB ... for unknown margaret olivero microbiology lab 1290 october 26 2013 identify the unknown organisms citrobacter freundii enterobacter aerogenes escherichia coli klebsiella Enterobacter aerogenes is a facultatively anaerobic gram negative rod that has peitrichous flagella. This reaction is observed as a white color in the medium. 2. Gas production as a side effect of fermentation was also possible, but clear results (cracks or bubbles in the medium) were difficult to see. Studies have found specific bacteria such as Enterobacter aerogenes are sensitive to melatonin rhythms. Drigalski-Conradi medium has also been used by several authors. April 18, 2019 by Sagar Aryal. Litmus milk test - alkaline orgs. nitrate reductase which results in the reduction of nitrate no3, biochemical test chart you will not fill in every blank in this chart alcaligenes faecalis bacillus megaterium bacillus subtilis enterobacter aerogenes enterobacter cloacae enterococcus faecalis escherichia coli ⦠(7) Nitrate Reduction. Decarboxylation Test. Biochemical Test Name. Both Proteus vulgaris and Pseudomonas aeruginosa are Gram-negative and rod-shaped. Litmus Milk Test Enterobacter aerogenes or any of hundreds of other species. There's a simple set up with consistent results. enterobacter aerogenes litmus milk test results E. After incubation the broth was still a yellow color, giving a negative result. Gas production as a side effect of fermentation was also possible, but clear results (cracks or bubbles in the medium) were difficult to see. considered a positive indol test particularly characteristic of Proteus vulgaris. In morphology, they are showing some differences in selective media, such as in Double violet agar medium. apr. Enterobacter species. In contrast, Enterobacter aerogenes and Klebsiella pneumoniae are MR- and VP+. Victor Nizet, Jerome O. Klein, in Infectious Diseases of the Fetus and Newborn (Seventh Edition), 2011. ... Enterobacter aerogenes: ... Tomato Juice/Yeast Extract/Milk Broth Litmus Milk Snyder Test Agar: Lactococcus lactis (Streptococcus lactis) Brain Heart Infusion Agar Tomato Juice/Yeast Extract/Milk Broth Snyder Test Agar February 4, 2021. laboratory diagnosis of mycoplasma pneumoniae infection. This confirmed that Enterobacter aerogenes was the gram-negative bacterium. 1. If litmus had been fermented, the milk turned pink (positive test). This bacteria is Gram-negative, rod-shaped, and radially surrounded by flagellum. The last experiment performed was a litmus milk broth test, performed by inoculating a lavender-colored tube and allowing it to incubate at 37°C. This is a bit tricky. This tests aids in the differentiation of the Leuconostoc species. Now nearing 40, plumpish and with her dancing days clearly behind her, Mata Hari fell in love with a 21-year-old Russian captain, Vladimir de Masloff, in 1916. Math. Ideal test to identify between Escherichia coli (E.coli) and Enterobacter aerogenes (EA). The following tests were performed on the Gram Positive bacterium: 1) Simmonâs Citrate. Languages. The Urea test was the final test. EBOOK CHARLESTONWESTSIDE PDF HTTP EBOOK. Enterococcus faecalis â formerly classified as part of the group D Streptococcus system â is a Gram-positive, commensal bacterium inhabiting the gastrointestinal tracts of humans. Litmus may also act as an electron acceptor thus becoming reduced by bacterial metabolism. The bacterium is approximately 1-3 microns in length, and is capable of motility via peritrichous flagella. Klebsiella pneumoniae Unknown Lab Report CPR St Louis. Lab Report Test identification of an unknown bacterium and writing up a report. Is salmonella a coliform? ⢠Enterobacter aerogenes + ⢠E. coli â (left) ⢠Barrittâs reagent Tests for acetoin, precursor to 2,3 butanediol fermentation ⢠Addition of alpha-napthol and KOH This is the beginning of the reaction, you should see a cherry red color throughout inoculation! Because of this situation, the ... Litmus Milk. Possible Results: By producing acid from the fermentation of lactose present in milk a bacterium may also cause the milk to curdle or clot in the bottom of the tube. All other results are negative. K. pneumoniae and Enterobacter aerogenes (K. mobilis) are most frequently involved, although K. oxytoca and R. planticola, and rarely R. terrigena, can be found. Basic Characteristics. (3) Indole Production. Test Procedure. The principal vectors are the hands of personnel [77,78]. 2. E. coli. Identifying Enterobacter aerogenes from a Mixed Culture of Unknown Gram Positive and Gram Negative Bacteria Kevin Le November 13, 2013 PURPOSE The focus of this study is to be able to identify an unknown bacterium, specifically a gram negative one,.from a mixed culture This study was done in order to apply the various lab techniques used to identify bacterium that were ⦠Klebsiella Pneumoniae Unknown Lab Report Test proteus vulgaris unknown lab report microbiology. Unknown 3 Microbiology Flashcards Quizlet. It tests for the bacteria speciesâ ability to produce indole. Table 1 contains the test and the recorded results for Staphylococcus epidermidis. IMViC Test. Just like the citrate test, itâs used to differentiate organisms in the Enterobacteriaceae family. lab results free essay sample new york essays, classification identification and clinical significance, escherichia coli uncovering unknown 72 jordyn kidd 4 23, proteus vulgaris microbiology sample essays, micro lab final all unknown bacteria flashcards quizlet, unknown lab report free essays phdessay Negative (-ve) Catalase. Perhaps it likes Litmus milk, but we wonât know until tomorrow because we forgot to do that sample (I know, I know⦠but give us a break! à¸à¸à¸à¸µà¹à¹à¸à¸µ à¸à¸à¸ªà¸à¸:: E. E. coli coli à¹à¸¥à¸° KlebsiellaKlebsiella aerogenes aerogenes ... Several different results in Litmus milk test Properties (Enterobacter cloacae) Capsule. K. aerogenes is a nosocomial and pathogenic bacterium that causes opportunistic ⦠The oxidase test was performed on unknown #122 gram positive, showed to be positive. Litmus Milk is used primarily to differentiate members within the genus Clostridium. It differentiates Enterobacteriaceae from other Gram-negative bacilli based on the ability of enterics to reduce litmus. If litmus had been fermented, the milk turned pink (positive test). A positive test results in the medium becoming pink after growth of the bacterium on the agar. Since hydrogen ion cannot exist in the Free State, there must be an acceptor of hydrogen ion. Methyl red test: Add 5 drops of methyl red to the remaining culture and examine the colour of each culture and note. The results from this test were negative which means there was no production of urease to break down urea and produce a pH change to trigger the phenol red to turn pink. microbiology lab molb 2210 laramie wyoming. (iv) Curd Formation: H 2S reacts w/ ferrous sulfate forming the Unknown Test Results S21 Updated.pdf - B rod fa B BG B(w \u03b3 General Key Litmus Milk Key Key to Fermentation Broth = positive result 0 = No Change an Properties (B. melitensis) Capsule. The result were as shown in the flow chart as well in the table, the determining step to figure out that it was gram negative was the urea and the MR test, which conformed that my gram negative bacteria was Enterobacter aerogenes. Starch test was performed; it showed a clearing on the agar, indicating a positive result. Example of an Unknown Lab Report in Microbiology BLS. This outcome eliminated Klebsiella pneumonia as a candidate. In the litmus milk test, litmus acts as the acceptor of the hydrogen ion. Litmus is purple in the oxidised state, but when it accepts hydrogen, it becomes reduced and turns white or milk-coloured. The biochemical activities of different bacteria grown in litmus milk may result in the production of two distinct types of curds (clots). industrial and agricultural wastes are increasingly ⢠Enterobacter aerogenes finding their way into public water supplies. In order to interpret the results of the starch hydrolysis test, iodine must be added to the agar. Purple is the only positive result and indicates that the organism produces the decarboxylase enzyme. eliminating klebsiella pneumoniae unknown lab Enzymatic Reactions. basic pH > 8.3; Subjects. Enterobacter aerogenes is a citrate positive where color of the medium changes from Green to blue while E. coli is a citrate negative where the medium remains green. The Voges-Proskauer test demonstrated a positive result indicating that Enterobacter aerogenes was the unknown bacterium. Enterobacter aerogenes, Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella typhimurium, Staphylococcus aureus. Litmus milk is simple sterilized skim milk with litmus added. Litmus milk medium is a selective medium that is used to differentiate Enterobacteriaceae members from the rest of the gram-negative bacteria present. Enterobacter aerogenes- alkaline. Eosin-Methylene Blue Agar medium â A Selective medium used to differentiate between lactose fermenters versus non-lactose fermenters. Sulfur reduction test, Indole production, Motility (SIM) deeps all 3 tests done w/SIM deeps just add Kovacâs reagent for Indole test ⢠Alcaligenes faecalis (left) - ⢠Escherichia coli (middle) â ⢠Proteus vulgaris (black precipitate) + ⢠Reagent: Ferrous ammonium sulfate-indicator. Principle The purpose of the litmus milk test is to determine the acidification and clot of the milk in this test. Test Detail Chronological Order. plate containing skim milk (a protein) that was inoculated with the unknown, allowed incubation at 35°C, and examined afterwards for growth around the bacteria (Lammert 2007). List of Biochemical Tests For Identification of Bacteria. Litmus Milk Test Results For Proteus Vulgaris Fullexams Com. CAMP Test. LITMUS MILK TEST RESULTS FOR PROTEUS VULGARIS FULLEXAMS COM. ... Microbiology Unknown Enterobacter aerogenes. So, we inoculated the Litmus milk and stuck it in the incubator. ... Enterobacter aerogenes and Klebsiella pneumoniae are MR- and VP+. The results were positive for urease, which eliminated all but Staphylococcus epidermidis as the Gram-positive unknown microorganism for the mixed culture of unknown 101. The development of a blue-purple color within a few seconds shows a positive result. Some of the characteristics are as follows: Fermentation of. This is an excellent growth medium and helpful in the characterization of unknown microorganisms. Objective of Litmus Milk Medium Test To differentiate microorganisms based on various metabolic reactions in litmus milk, including fermentation, reduction, clot formation, digestion, and the formation of gas. There's a simple set up with consistent results. Klebsiella aerogenes, previously known as Enterobacter aerogenes, is a Gram-negative, oxidase negative, catalase positive, citrate positive, indole negative, rod-shaped bacterium. Property it tests for: This test is performed to help differentiate species of the family Enterobacteriaceae. Incubate inoculated plate aerobically at 37°C. Ten fitted closely the description of either A. aerogenes or E. coli, as described in Bergey's Manual. Proteus Vulgaris Unknown Lab Report Painting E4gle Org. 4. Klebsiella pneumoniae showing - ⦠unknown lab essay â free papers and essays examples. Horowitz, in part of his work, used a gelatin litmus lactose medium. List of Biochemical Tests. Nitrate ⦠Positive (+ve) Phenylalanine Deaminase Test This is a synergistic test between Staphylococcus aureus and Streptococcus agalactiae. Colony of the isolates was smear on the filter paper. As melatonin is secreted from the gut lining, it can cause an increase in Enterobacter aerogenes. K aerogenes should show blue and a ' + result. When used alone, the IMViC tests are particularly useful for differentiating Escherichia coli, Enterobacter aerogenes, Enterobacter cloacae, and Klebsiella pneumoniae (although colonial morphology and the presence of capsules can also be used to ⦠âColiformsâ include E. coli, Enterobacter aerogenes, Citrobacter, and Klebsiella. Table of Contents. Positive (+ve) To differentiate among microorganisms that enzymatically transforms different milk substrates into varied metabolic end products. Types of Reactions Seen with Litmus Milk Medium (5) Voges-Proskauer Test [VP-Test] (6) Citrate Utilization. The probiotic strains such as Symbioflor1 and EF-2001 are characterized by the lack ⦠Tests for lactose fermentation, reduction of litmus, presence of caseinase, deamination of amino acids to produce NH3 ... Eschericia coli and Enterobacter aerogenes . https://microbewiki.kenyon.edu/index.php/Enterobacter_aerogenes -Both Klebsiella pneumoniae and Enterobacter aerogenes will reduce the litmus, resulting in the bottom to change into a white color. March 25, 2021 March 24, 2021 by Biocheminsider. EXPECTED RESULTS: Tubes displaying gas in the Durham tube accompanied by a yellow color of the medium represent a presumptive positive test for coliforms in the test specimen. EMB Plate Results: Staphylococcus epidermidis (no growth), E. coli (dark purple), Pseudomonas aeruginosa (bottom), Enterobacter aerogenes (left) . ... alkaline conditions). Biochemical Test of Enterobacter aerogenes. Enterobacter aerogenes. identification of an unknown bacterium and writing up a report. identification of unknown bacteria by myriam feldman issuu. enterobacter aerogenes. ... What does litmus milk test for? E. aerogenes is closely related to a wide range of other common microorganisms including Escherichia, Klebsiella, Shigella, and Serratia . Inoculate litmus milk medium with 4 drops of a 24-hour broth culture. Litmus Milk Test Klebsiella Pneumoniae fullexams com. The IMViC tests are useful for differentiating the Enterobacteriaceae, especially when used alongside the urease test. Is Enterobacter aerogenes a coliform? Arts and Humanities. Klebsiella is among the five gram-negative pathogens most commonly encountered in hospital-acquired infections (), and Klebsiella pneumoniae is the most frequently occurring species, accounting for 75 to 86% of Klebsiella species reported (3, 10, 29).Much more rarely encountered are Klebsiella ozaenae and Klebsiella rhinoscleromatis, which have been retained as separate ⦠... Enterobacter aerogenes: ... Tomato Juice/Yeast Extract/Milk Broth Litmus Milk Snyder Test Agar: Lactococcus lactis (Streptococcus lactis) Brain Heart Infusion Agar Tomato Juice/Yeast Extract/Milk Broth Snyder Test Agar unknown lab report sample essays. Microbiology 20 Biochemical Unknown â Fall 2008. Litmus Milk test. Structure and Physiology. Oxidase Test A piece of filter paper was placed in a clean Petri dish and 2 drops of freshly prepared oxidase reagent was added using a wire loop. Litmus is purple in the oxidised state, but when it accepts hydrogen, it becomes reduced and turns white or milk-coloured. Prussian blue is and green is. identifying unknown bacteria essay example for free. positive. I will recommend this book to 2 of my best friends, and I hope it will be as valuable to them as it was for me. It is a motile lactose fermenter. Biochemical Test of Brucella melitensis. Procedure of Litmus Milk Medium Test. The eosin methylene blue agar (EMB agar) is a selective staining for gram-negative bacteria. tions. How To ... IDENTIFY YOUR UNKNOWN CULTURES BASED ON THE RESULTS OF THIS LAB' 4 / 43 'LAB TESTS FOR KLEBSIELLA PNEUMONIAE FULLEXAMS COM JANUARY 15TH, 2018 - KLEBSIELLA PNEUMONIAE IN URINE CULTURE MORE LAB TESTS FOR KLEBSIELLA PNEUMONIAE HOW ... 'Microbiology Unknown ⦠Bacteria use an enzyme, tryptophanase to break down the amino acid, tryptophan, which makes by-products, of which, indole is one. ... reaction observed may occasionally be due to the question are inoculated in litmus milk medium, and ... nique to ⦠Proteus Vulgaris Microbiology 2931 Words Bartleby. Incubate at 37°C in ambient air for 24-48 hours. pH colors for Litmus milk test At an acidic pH the color is pink (red) (around pH of 5), and blue at an alkaline pH (around pH of 8). 12. Antimicrobial susceptibility of urinary Klebsiella. 2) Maltose. Synopsis. Klebsiella pneumoniae Wikipedia. Pseudomonas aeruginosa is a glucose nonfermenter and is thus MR- and VP-. The iodine reacts with the starch to form a dark brown color. Enterobacter in the human microbiota is a pathogenic (=disease-causing) organism or is capable of becoming pathogenic. proteus vulgaris unknown lab report microbiology. Oxidase test Principle: Cytochrome oxidase Indicator/Reagent: Indophenols blue Screening test Filter paper method - Tetramethyl p-phenylene diamine dihydrochloride (+) Bluish purple (+) Pseudomonas aeruginosa (-) Escherichia coli 2. Others included one each of E. freun- dii, E. intermedia~ P. aerogenoides, and an A. aerogenes that gave a positive methyl red test but still classified as an A. aerogenes. Pseudomonas aeruginosa. E. coli - note metallic sheen = lactose fermentor Litmus Milk Test. Litmus milk test - acid pH. Like other species in the genus Enterococcus, E. faecalis is found in healthy humans and can be used as a probiotic. Harrybrownsportfolio Weebly Com. 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