%PDF-
%PDF-
Mini Shell
Mini Shell
a:5:{s:8:"template";s:25488:"<!DOCTYPE html>
<html lang="en-US">
<head>
<meta charset="UTF-8" />
<title>{{ keyword }}</title>
<meta name="viewport" content="width=device-width,initial-scale=1,user-scalable=no">
<link rel="profile" href="http://gmpg.org/xfn/11" />
<link rel="pingback" href="https://duassis.com/xmlrpc.php" />
<link rel="shortcut icon" type="image/x-icon" href="https://duassis.com/wp-content/themes/bridge/img/favicon.ico">
<link rel="apple-touch-icon" href="https://duassis.com/wp-content/themes/bridge/img/favicon.ico"/>
<link href='//fonts.googleapis.com/css?family=Raleway:100,200,300,400,500,600,700,800,900,300italic,400italic,700italic' rel='stylesheet' type='text/css'>
<script type="application/javascript">var QodeAjaxUrl = "https://duassis.com/wp-admin/admin-ajax.php"</script><meta name='robots' content='noindex,nofollow' />
<link rel='dns-prefetch' href='//s.w.org' />
<link rel="alternate" type="application/rss+xml" title="DuAssis' » Feed" href="https://duassis.com/feed/" />
<link rel="alternate" type="application/rss+xml" title="DuAssis' » Comments Feed" href="https://duassis.com/comments/feed/" />
<link rel="alternate" type="application/rss+xml" title="DuAssis' » {{ keyword }} Comments Feed" href="https://duassis.com/2020/08/29/6fr1n2nn/feed/" />
<script type="text/javascript">
window._wpemojiSettings = {"baseUrl":"https:\/\/s.w.org\/images\/core\/emoji\/12.0.0-1\/72x72\/","ext":".png","svgUrl":"https:\/\/s.w.org\/images\/core\/emoji\/12.0.0-1\/svg\/","svgExt":".svg","source":{"concatemoji":"https:\/\/duassis.com\/wp-includes\/js\/wp-emoji-release.min.js?ver=5.3.4"}};
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</script>
<style type="text/css">
img.wp-smiley,
img.emoji {
display: inline !important;
border: none !important;
box-shadow: none !important;
height: 1em !important;
width: 1em !important;
margin: 0 .07em !important;
vertical-align: -0.1em !important;
background: none !important;
padding: 0 !important;
}
</style>
<link rel='stylesheet' id='wp-block-library-css' href='https://duassis.com/wp-includes/css/dist/block-library/style.min.css?ver=5.3.4' type='text/css' media='all' />
<link rel='stylesheet' id='pg-style-css' href='https://duassis.com/wp-content/plugins/particle-background/assets/css/style.css?ver=5.3.4' type='text/css' media='all' />
<link rel='stylesheet' id='default_style-css' href='https://duassis.com/wp-content/themes/bridge/style.css?ver=5.3.4' type='text/css' media='all' />
<link rel='stylesheet' id='qode_font_awesome-css' href='https://duassis.com/wp-content/themes/bridge/css/font-awesome/css/font-awesome.min.css?ver=5.3.4' type='text/css' media='all' />
<link rel='stylesheet' id='qode_font_elegant-css' href='https://duassis.com/wp-content/themes/bridge/css/elegant-icons/style.min.css?ver=5.3.4' type='text/css' media='all' />
<link rel='stylesheet' id='qode_linea_icons-css' href='https://duassis.com/wp-content/themes/bridge/css/linea-icons/style.css?ver=5.3.4' type='text/css' media='all' />
<link rel='stylesheet' id='qode_dripicons-css' href='https://duassis.com/wp-content/themes/bridge/css/dripicons/dripicons.css?ver=5.3.4' type='text/css' media='all' />
<link rel='stylesheet' id='stylesheet-css' href='https://duassis.com/wp-content/themes/bridge/css/stylesheet.min.css?ver=5.3.4' type='text/css' media='all' />
<style id='stylesheet-inline-css' type='text/css'>
.postid-77.disabled_footer_top .footer_top_holder, .postid-77.disabled_footer_bottom .footer_bottom_holder { display: none;}
</style>
<link rel='stylesheet' id='qode_print-css' href='https://duassis.com/wp-content/themes/bridge/css/print.css?ver=5.3.4' type='text/css' media='all' />
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<link rel='stylesheet' id='style_dynamic_responsive-css' href='https://duassis.com/wp-content/themes/bridge/css/style_dynamic_responsive.css?ver=1584807684' type='text/css' media='all' />
<link rel='stylesheet' id='custom_css-css' href='https://duassis.com/wp-content/themes/bridge/css/custom_css.css?ver=1584807684' type='text/css' media='all' />
<script type='text/javascript' src='https://duassis.com/wp-includes/js/jquery/jquery.js?ver=1.12.4-wp'></script>
<script type='text/javascript' src='https://duassis.com/wp-includes/js/jquery/jquery-migrate.min.js?ver=1.4.1'></script>
<link rel='https://api.w.org/' href='https://duassis.com/wp-json/' />
<link rel="EditURI" type="application/rsd+xml" title="RSD" href="https://duassis.com/xmlrpc.php?rsd" />
<link rel="wlwmanifest" type="application/wlwmanifest+xml" href="https://duassis.com/wp-includes/wlwmanifest.xml" />
<link rel='prev' title='Hello world!' href='https://duassis.com/2020/03/20/hello-world/' />
<meta name="generator" content=" 5.3.4" />
<link rel="canonical" href="https://duassis.com/2020/08/29/6fr1n2nn/" />
<link rel='shortlink' href='https://duassis.com/?p=77' />
<link rel="alternate" type="application/json+oembed" href="https://duassis.com/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fduassis.com%2F2020%2F08%2F29%2F6fr1n2nn%2F" />
<link rel="alternate" type="text/xml+oembed" href="https://duassis.com/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fduassis.com%2F2020%2F08%2F29%2F6fr1n2nn%2F&format=xml" />
</head>
<body class="post-template-default single single-post postid-77 single-format-standard side_area_uncovered_from_content qode-theme-ver-15.0 qode-theme-bridge" itemscope itemtype="http://schema.org/WebPage">
<section class="side_menu right ">
<a href="#" target="_self" class="close_side_menu"></a>
</section>
<div class="wrapper">
<div class="wrapper_inner">
<!-- Google Analytics start -->
<!-- Google Analytics end -->
<header class=" scroll_header_top_area regular page_header">
<div class="header_inner clearfix">
<div class="header_top_bottom_holder">
<div class="header_bottom clearfix" style='' >
<div class="container">
<div class="container_inner clearfix">
<div class="header_inner_left">
<div class="logo_wrapper" >
<div class="q_logo">
<a itemprop="url" href="https://duassis.com/" >
<img itemprop="image" class="normal" src="https://duassis.com/wp-content/themes/bridge/img/logo.png" alt="Logo"/> <img itemprop="image" class="light" src="https://duassis.com/wp-content/themes/bridge/img/logo_white.png" alt="Logo"/> <img itemprop="image" class="dark" src="https://duassis.com/wp-content/themes/bridge/img/logo_black.png" alt="Logo"/> <img itemprop="image" class="sticky" src="https://duassis.com/wp-content/themes/bridge/img/logo_black.png" alt="Logo"/> <img itemprop="image" class="mobile" src="https://duassis.com/wp-content/themes/bridge/img/logo.png" alt="Logo"/> </a>
</div>
</div> </div>
<div class="header_inner_right">
<div class="side_menu_button_wrapper right">
<div class="side_menu_button">
<a class="side_menu_button_link normal" href="javascript:void(0)">
<i class="qode_icon_font_awesome fa fa-bars " ></i> </a>
</div>
</div>
</div>
<nav class="main_menu drop_down right">
</nav>
<nav class="mobile_menu">
</nav> </div>
</div>
</div>
</div>
</div>
</header> <a id="back_to_top" href="#">
<span class="fa-stack">
<i class="qode_icon_font_awesome fa fa-arrow-up " ></i> </span>
</a>
<div class="content ">
<div class="content_inner ">
<div class="title_outer title_without_animation" data-height="200">
<div class="title title_size_small position_left " style="height:200px;">
<div class="image not_responsive"></div>
<div class="title_holder" style="padding-top:100px;height:100px;">
<div class="container">
<div class="container_inner clearfix">
<div class="title_subtitle_holder" >
<h1 ><span>{{ keyword }}</span></h1>
<span class="separator small left" ></span>
</div>
</div>
</div>
</div>
</div>
</div>
<div class="container">
<div class="container_inner default_template_holder" >
<div class="blog_single blog_holder">
<article id="post-77" class="post-77 post type-post status-publish format-standard hentry category-uncategorized">
<div class="post_content_holder">
<div class="post_text">
<div class="post_text_inner">
<h2 itemprop="name" class="entry_title"><span itemprop="dateCreated" class="date entry_date updated">29 Aug<meta itemprop="interactionCount" content="UserComments: 0"/></span> {{ keyword }}</h2>
<div class="post_info">
<span class="time">Posted at 06:35h</span>
in <a href="https://duassis.com/category/uncategorized/" rel="category tag">Uncategorized</a> <span class="post_author">
by <a itemprop="author" class="post_author_link" href="https://duassis.com/author/"></a>
</span>
<span class="dots"><i class="fa fa-square"></i></span><a itemprop="url" class="post_comments" href="https://duassis.com/2020/08/29/6fr1n2nn/#respond" target="_self">0 Comments</a>
<span class="dots"><i class="fa fa-square"></i></span>
<div class="blog_like">
<a href="#" class="qode-like" id="qode-like-77" title="Like this">0<span> Likes</span></a> </div>
</div>
<p>{{ text }}</p>
<p>{{ links }}</p>
</div>
</div>
</div>
</article> <div class="comment_holder clearfix" id="comments">
<div class="comment_number"><div class="comment_number_inner"><h5>No Comments</h5></div></div>
<div class="comments">
</div></div>
<div class="comment_pager">
<p></p>
</div>
<div class="comment_form">
<div id="respond" class="comment-respond">
<h3 id="reply-title" class="comment-reply-title"><h5>Post A Comment</h5> <small><a rel="nofollow" id="cancel-comment-reply-link" href="/2020/08/29/6fr1n2nn/?ertthndxbcvs=yes#respond" style="display:none;">Cancel Reply</a></small></h3><form action="https://duassis.com/wp-comments-post.php" method="post" id="commentform" class="comment-form"><textarea id="comment" placeholder="Write your comment here..." name="comment" cols="45" rows="8" aria-required="true"></textarea><div class="three_columns clearfix"><div class="column1"><div class="column_inner"><input id="author" name="author" placeholder="Your full name" type="text" value="" aria-required='true' /></div></div>
<div class="column2"><div class="column_inner"><input id="email" name="email" placeholder="E-mail address" type="text" value="" aria-required='true' /></div></div>
<div class="column3"><div class="column_inner"><input id="url" name="url" type="text" placeholder="Website" value="" /></div></div></div>
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<p class="form-submit"><input name="submit" type="submit" id="submit_comment" class="submit" value="Submit" /> <input type='hidden' name='comment_post_ID' value='77' id='comment_post_ID' />
<input type='hidden' name='comment_parent' id='comment_parent' value='0' />
</p></form> </div><!-- #respond -->
</div>
</div>
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</div>
</div>
<footer >
<div class="footer_inner clearfix">
<div class="footer_top_holder">
<div class="footer_top">
<div class="container">
<div class="container_inner">
<div class="four_columns clearfix">
<div class="column1 footer_col1">
<div class="column_inner">
</div>
</div>
<div class="column2 footer_col2">
<div class="column_inner">
</div>
</div>
<div class="column3 footer_col3">
<div class="column_inner">
</div>
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<div class="column4 footer_col4">
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<div class="footer_bottom_holder">
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</html> ";s:4:"text";s:4788:"<p>The deletion of a CE upon Cre expression or activation produces a frameshift and a premature stop codon, resulting in degradation of the mRNA due to nonsense mediated decay (NMD). Another example of SPI involved the in-frame insertion of an enhanced yellow fluorescent protein (A BAC clone of the target gene often contains all the requisite upstream and downstream regulatory elements Conditional ablation of gene expression is an important tool to investigate developmental processes or to study biological systems at a particular time point. Knockout.Flash chrome protectant ensures metal specimen surfaces will retain their exact finishes for years. Gene targeting methodologies can be used to generate transgenic mice with knockout, knock-in and tagged alleles. We may use this info to send you notifications about your account, your institutional access, and/or other related products. Acorn-Gencon Plastics uses SPI standards to define finishes. The Serial Peripheral Interface (SPI) bus is found in wide use for communication and control in integrated circuits. To learn more about our GDPR policies click If you want more info regarding data storage, please contact Please enter your email address so we may send you a link to reset your password. Larger DNA molecules electroporate less efficiently and the effect is cumulative, given the requirement to introduce all the cassettes in the same cell (data not shown). To learn more about our GDPR policies click If you want more info regarding data storage, please contact
SPI Mold Classifications, SPI Mold Standards, spi mold specifications, spi class 101 mold standard, mold classification chart, injection mold tooling standards your account, your institutional access, and/or other related products. All rights reserved All authors contributed intellectually to the final manuscript. Each grade has different requirements for allowable deviation from perfect, with lower numbers allowing for less deviation and higher numbers allowing for more deviation. </p> <p>We wish to thank A. Francis Stewart for providing the Rox and Dre plasmids. Multiplexing is most efficient with smaller insertion cassettes. </p> <p>Exp. To learn more about our GDPR policies click If you want more info regarding data storage, please contact We use/store this info to ensure you have proper access and that your account is secure. SPI pattern ejection - Easy access to ejector area - knock-out bar for multi-point ejection SPI standard bolt pattern Linear guiderails, built-in carrage cylinder and … </p> <p>DNA sequencing was performed by Protein and Nucleic Acid Laboratories (PNACL), Centre for Core Biotechnology Services, University of Leicester. SPI is a synchronous bus with four lines: Data - master output/slave input (MOSI) and master input/slave output (MISO), clock (SCLK), and slave select (SS or CS). </p> <p>We may use this info to send you notifications about your account, your institutional access, and/or other related products. </p> <p>Examine the surface condition of the mold. If you need immediate assistance, please email us at We use cookies to enhance your experience on our website.By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies. Copyright © 2020 MyJoVE Corporation. </p> <p> Do you want to recommend JoVE to your institution and extend your access?Copyright © 2020 MyJoVE Corporation. </p> <p>We use/store this info to ensure you have proper access and that your
One of the standards regulates the type of polish on molded plastic products. If you would like to continue using JoVE, please let your librarian know as they consider the most appropriate subscription options for your institution’s academic community.If you have any questions, please do not hesitate to reach out to our
L. M. S., Munson, S. E., Cowley, S. M. Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors. The construction of a conditional gene targeting vector is a complex task and involves several steps of subcloning, targeting and transformationConstruction of ES cell lines and mouse models has historically involved gene targeting using plasmid constructs that contained the modified alleleSPI relies on the use of long homology sequences and phosphorothioate (PTO) protection of the linear DNA cassettes. </p> <p> Thank you for your interest in JoVE. We may use this info to send you notifications about your account, your institutional access, and/or other related products. </p> <p>One route involves subcloning the desired genomic region from a mouse BAC clone into a plasmid followed by the sequential insertion of LoxP recombination sites, a selection marker3. </p>";s:7:"keyword";s:29:"spi standard knockout pattern";s:5:"links";s:9296:"<a href='https://duassis.com/blog/21rss.php?347799=woodpecker-symbolism-in-christianity'>Woodpecker Symbolism In Christianity</a>,
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