%PDF- %PDF-
Mini Shell

Mini Shell

Direktori : /var/www/html/shaban/duassis/api/public/storage/xwqtu/cache/
Upload File :
Create Path :
Current File : /var/www/html/shaban/duassis/api/public/storage/xwqtu/cache/047444d543573b3e2e8b8c1f4fac996b

a:5:{s:8:"template";s:9437:"<!DOCTYPE html>
<html lang="en">
<head>
<meta charset="utf-8"/>
<meta content="width=device-width, initial-scale=1.0" name="viewport"/>
<title>{{ keyword }}</title>
<link href="//fonts.googleapis.com/css?family=Open+Sans%3A300%2C400%2C600%2C700%2C800%7CRoboto%3A100%2C300%2C400%2C500%2C600%2C700%2C900%7CRaleway%3A600%7Citalic&amp;subset=latin%2Clatin-ext" id="quality-fonts-css" media="all" rel="stylesheet" type="text/css"/>
<style rel="stylesheet" type="text/css"> html{font-family:sans-serif;-webkit-text-size-adjust:100%;-ms-text-size-adjust:100%}body{margin:0}footer,nav{display:block}a{background:0 0}a:active,a:hover{outline:0}@media print{*{color:#000!important;text-shadow:none!important;background:0 0!important;box-shadow:none!important}a,a:visited{text-decoration:underline}a[href]:after{content:" (" attr(href) ")"}a[href^="#"]:after{content:""}p{orphans:3;widows:3}.navbar{display:none}}*{-webkit-box-sizing:border-box;-moz-box-sizing:border-box;box-sizing:border-box}:after,:before{-webkit-box-sizing:border-box;-moz-box-sizing:border-box;box-sizing:border-box}html{font-size:62.5%;-webkit-tap-highlight-color:transparent}body{font-family:"Helvetica Neue",Helvetica,Arial,sans-serif;font-size:14px;line-height:1.42857143;color:#333;background-color:#fff}a{color:#428bca;text-decoration:none}a:focus,a:hover{color:#2a6496;text-decoration:underline}a:focus{outline:thin dotted;outline:5px auto -webkit-focus-ring-color;outline-offset:-2px}p{margin:0 0 10px}ul{margin-top:0;margin-bottom:10px}.container{padding-right:15px;padding-left:15px;margin-right:auto;margin-left:auto}@media (min-width:768px){.container{width:750px}}@media (min-width:992px){.container{width:970px}}@media (min-width:1200px){.container{width:1170px}}.container-fluid{padding-right:15px;padding-left:15px;margin-right:auto;margin-left:auto}.row{margin-right:-15px;margin-left:-15px}.col-md-12{position:relative;min-height:1px;padding-right:15px;padding-left:15px}@media (min-width:992px){.col-md-12{float:left}.col-md-12{width:100%}}.collapse{display:none} .nav{padding-left:0;margin-bottom:0;list-style:none}.nav>li{position:relative;display:block}.nav>li>a{position:relative;display:block;padding:10px 15px}.nav>li>a:focus,.nav>li>a:hover{text-decoration:none;background-color:#eee}.navbar{position:relative;min-height:50px;margin-bottom:20px;border:1px solid transparent}@media (min-width:768px){.navbar{border-radius:4px}}@media (min-width:768px){.navbar-header{float:left}}.navbar-collapse{max-height:340px;padding-right:15px;padding-left:15px;overflow-x:visible;-webkit-overflow-scrolling:touch;border-top:1px solid transparent;box-shadow:inset 0 1px 0 rgba(255,255,255,.1)}@media (min-width:768px){.navbar-collapse{width:auto;border-top:0;box-shadow:none}.navbar-collapse.collapse{display:block!important;height:auto!important;padding-bottom:0;overflow:visible!important}}.container-fluid>.navbar-collapse,.container-fluid>.navbar-header{margin-right:-15px;margin-left:-15px}@media (min-width:768px){.container-fluid>.navbar-collapse,.container-fluid>.navbar-header{margin-right:0;margin-left:0}}.navbar-brand{float:left;height:50px;padding:15px 15px;font-size:18px;line-height:20px}.navbar-brand:focus,.navbar-brand:hover{text-decoration:none}@media (min-width:768px){.navbar>.container-fluid .navbar-brand{margin-left:-15px}}.navbar-nav{margin:7.5px -15px}.navbar-nav>li>a{padding-top:10px;padding-bottom:10px;line-height:20px}@media (min-width:768px){.navbar-nav{float:left;margin:0}.navbar-nav>li{float:left}.navbar-nav>li>a{padding-top:15px;padding-bottom:15px}.navbar-nav.navbar-right:last-child{margin-right:-15px}}@media (min-width:768px){.navbar-right{float:right!important}}.clearfix:after,.clearfix:before,.container-fluid:after,.container-fluid:before,.container:after,.container:before,.nav:after,.nav:before,.navbar-collapse:after,.navbar-collapse:before,.navbar-header:after,.navbar-header:before,.navbar:after,.navbar:before,.row:after,.row:before{display:table;content:" "}.clearfix:after,.container-fluid:after,.container:after,.nav:after,.navbar-collapse:after,.navbar-header:after,.navbar:after,.row:after{clear:both}@-ms-viewport{width:device-width}html{font-size:14px;overflow-y:scroll;overflow-x:hidden;-ms-overflow-style:scrollbar}@media(min-width:60em){html{font-size:16px}}body{background:#fff;color:#6a6a6a;font-family:"Open Sans",Helvetica,Arial,sans-serif;font-size:1rem;line-height:1.5;font-weight:400;padding:0;background-attachment:fixed;text-rendering:optimizeLegibility;overflow-x:hidden;transition:.5s ease all}p{line-height:1.7;margin:0 0 25px}p:last-child{margin:0}a{transition:all .3s ease 0s}a:focus,a:hover{color:#121212;outline:0;text-decoration:none}.padding-0{padding-left:0;padding-right:0}ul{font-weight:400;margin:0 0 25px 0;padding-left:18px}ul{list-style:disc}ul>li{margin:0;padding:.5rem 0;border:none}ul li:last-child{padding-bottom:0}.site-footer{background-color:#1a1a1a;margin:0;padding:0;width:100%;font-size:.938rem}.site-info{border-top:1px solid rgba(255,255,255,.1);padding:30px 0;text-align:center}.site-info p{color:#adadad;margin:0;padding:0}.navbar-custom .navbar-brand{padding:25px 10px 16px 0}.navbar-custom .navbar-nav>li>a:focus,.navbar-custom .navbar-nav>li>a:hover{color:#f8504b}a{color:#f8504b}.navbar-custom{background-color:transparent;border:0;border-radius:0;z-index:1000;font-size:1rem;transition:background,padding .4s ease-in-out 0s;margin:0;min-height:100px}.navbar a{transition:color 125ms ease-in-out 0s}.navbar-custom .navbar-brand{letter-spacing:1px;font-weight:600;font-size:2rem;line-height:1.5;color:#121213;margin-left:0!important;height:auto;padding:26px 30px 26px 15px}@media (min-width:768px){.navbar-custom .navbar-brand{padding:26px 10px 26px 0}}.navbar-custom .navbar-nav li{margin:0 10px;padding:0}.navbar-custom .navbar-nav li>a{position:relative;color:#121213;font-weight:600;font-size:1rem;line-height:1.4;padding:40px 15px 40px 15px;transition:all .35s ease}.navbar-custom .navbar-nav>li>a:focus,.navbar-custom .navbar-nav>li>a:hover{background:0 0}@media (max-width:991px){.navbar-custom .navbar-nav{letter-spacing:0;margin-top:1px}.navbar-custom .navbar-nav li{margin:0 20px;padding:0}.navbar-custom .navbar-nav li>a{color:#bbb;padding:12px 0 12px 0}.navbar-custom .navbar-nav>li>a:focus,.navbar-custom .navbar-nav>li>a:hover{background:0 0;color:#fff}.navbar-custom li a{border-bottom:1px solid rgba(73,71,71,.3)!important}.navbar-header{float:none}.navbar-collapse{border-top:1px solid transparent;box-shadow:inset 0 1px 0 rgba(255,255,255,.1)}.navbar-collapse.collapse{display:none!important}.navbar-custom .navbar-nav{background-color:#1a1a1a;float:none!important;margin:0!important}.navbar-custom .navbar-nav>li{float:none}.navbar-header{padding:0 130px}.navbar-collapse{padding-right:0;padding-left:0}}@media (max-width:768px){.navbar-header{padding:0 15px}.navbar-collapse{padding-right:15px;padding-left:15px}}@media (max-width:500px){.navbar-custom .navbar-brand{float:none;display:block;text-align:center;padding:25px 15px 12px 15px}}@media (min-width:992px){.navbar-custom .container-fluid{width:970px;padding-right:15px;padding-left:15px;margin-right:auto;margin-left:auto}}@media (min-width:1200px){.navbar-custom .container-fluid{width:1170px;padding-right:15px;padding-left:15px;margin-right:auto;margin-left:auto}} @font-face{font-family:'Open Sans';font-style:normal;font-weight:300;src:local('Open Sans Light'),local('OpenSans-Light'),url(http://fonts.gstatic.com/s/opensans/v17/mem5YaGs126MiZpBA-UN_r8OXOhs.ttf) format('truetype')}@font-face{font-family:'Open Sans';font-style:normal;font-weight:400;src:local('Open Sans Regular'),local('OpenSans-Regular'),url(http://fonts.gstatic.com/s/opensans/v17/mem8YaGs126MiZpBA-UFW50e.ttf) format('truetype')} @font-face{font-family:Roboto;font-style:normal;font-weight:700;src:local('Roboto Bold'),local('Roboto-Bold'),url(http://fonts.gstatic.com/s/roboto/v20/KFOlCnqEu92Fr1MmWUlfChc9.ttf) format('truetype')}@font-face{font-family:Roboto;font-style:normal;font-weight:900;src:local('Roboto Black'),local('Roboto-Black'),url(http://fonts.gstatic.com/s/roboto/v20/KFOlCnqEu92Fr1MmYUtfChc9.ttf) format('truetype')} </style>
 </head>
<body class="">
<nav class="navbar navbar-custom" role="navigation">
<div class="container-fluid padding-0">
<div class="navbar-header">
<a class="navbar-brand" href="#">
{{ keyword }}
</a>
</div>
<div class="collapse navbar-collapse" id="custom-collapse">
<ul class="nav navbar-nav navbar-right" id="menu-menu-principale"><li class="menu-item menu-item-type-post_type menu-item-object-post menu-item-169" id="menu-item-169"><a href="#">About</a></li>
<li class="menu-item menu-item-type-post_type menu-item-object-post menu-item-121" id="menu-item-121"><a href="#">Location</a></li>
<li class="menu-item menu-item-type-post_type menu-item-object-post menu-item-120" id="menu-item-120"><a href="#">Menu</a></li>
<li class="menu-item menu-item-type-post_type menu-item-object-post menu-item-119" id="menu-item-119"><a href="#">FAQ</a></li>
<li class="menu-item menu-item-type-post_type menu-item-object-post menu-item-122" id="menu-item-122"><a href="#">Contacts</a></li>
</ul> </div>
</div>
</nav>
<div class="clearfix"></div>
{{ text }}
<br>
{{ links }}
<footer class="site-footer">
<div class="container">
<div class="row">
<div class="col-md-12">
<div class="site-info">
<p>{{ keyword }} 2021</p></div>
</div>
</div>
</div>
</footer>
</body>
</html>";s:4:"text";s:14777:"Or, as Dr. Ruchi Mathur sometimes calls it, "that little guy." e) sensing nutrient in the environment. Halobacterium salinarum S9 cells grown to a density of ∼7×10 8 cells/ml were pelleted for 45 min at 6000 g and resuspended in transport buffer, TBS [halobacterial complete medium (Oesterhelt and Stoeckenius, 1974) without citrate and peptone, buffered with 50 mM HEPES pH 7.0] to a density of 0.25×10 9 cells/ml. Show transcribed image text. hot springs. They can live in extreme environments. Particularly, bacteriorhodopsins have been used in holographic storage, optical switching, motion detection, and nanotechnology. Halobacteria are photoheterotrophic organisms, meaning that they use light for energy, but can't use carbon dioxide as their sole carbon source. No they are not eukariyotic.They are prokariyotic organisms. It is found in salted fish, hides, hypersaline lakes, and salterns. The purple Halobacteria is caused by a light sensitive protein called bacteriarhodopsin, which uses sunlight to drive protons out of the cell, giving the cell energy. a Mean square displacement <u²> for large amplitude fluctuations plotted against temperature for unstressed and low-salt-stressed cells. SP1 23. Are halobacteria eukaryotic? It will swim forward, pause, and move backwards. Strain Halobacterium salinarum ST 3 obtained by a multistage breeding strain of halophilic bacteria Halobacterium salinarum VKPM b-10425 without pre-treatment with chemical reagents and deposited in PMBC under the registration number In-11850. It is only stabilized in the presence of a high sodium cation concentration. Halobacterium sp. • One of the … Halobacterium salinarum is an extremely halophilic archaeon that is widely distributed in hypersaline environments and was originally isolated as a spoilage organism of salted fish and hides. Bio review #7. The different changes in stimuli such as the chemical environment and light reactivity is when the archaean will switch its movement. It accumulates molar concentrations of KCl in the cytosol to counterbalance the external osmotic pressure imposed by the molar NaCl. This mesophilic microbe is a Gram-negative, rod shaped into a single arrangement which is significant in their ability to move and survive in the harsh environment they live in. M. smithii for short. How does it move- … b) protection from high salt concentrations. H. salinarum are co-cultured with R. sphaeroides to determine the impact of purple membrane fragments (PM) on R. sphaeroides and improve its hydrogen production capacity. It is rod-shaped, motile, lives in highly saline environments (4M salt and higher), and is one of the few species known that can live in saturated salt solutions. Gas vesicles increase the buoyancy of cells and enable them to migrate … As a consequence, cytosolic proteins are permanently exposed to low water activity and highly ionic … Class- Halobacteria. It's all right for them to be on a nickname-basis. 24. Not only does this give insight into the origin of life on Earth, but opens up a new realm of possibilities for life elsewhere in the universe. How does halobacteria protect itself from destruction by UV light? 89 terms. Some Archaebacteria can also be heterotrophs. Halobacterium are in the domain of Archea, a group of single-celled micro-organisms, and are therefore not bacteria. Also Know, what does halobacterium Salinarum look like? Ectoine Ectoine is commercially produced by extracting the compound from halophilic bacteria. The genome of Halobacterium salinarum encodes four proteins of the structural maintenance of chromosomes (SMC) protein superfamily. … They are known to live in intense environments with high salinity, intense illumination, and oxygen limitation. Halobacterium salinarum needs very little to survive, but a Growth Medium is vital because it will be acting as a salty environment and provide nutrients for growth. Species- H. salinarium. Other articles where Halobacterium is discussed: bacteria: 16S rRNA analysis: The only photosynthetic archaeon, Halobacterium, has a completely different type of photosynthesis that does not use chlorophyll in large protein complexes to activate an electron, as in plants and bacteria. Halobacterium salinarum. Halobacterium salinarum does not utilize simple nitrogen sources; for growth it relies on the … However, the effect of environmental stress factors like heavy metals on biofilms was still unknown. What does it eat- nothing. Order- Halobacteriales. They maintain high NaCl concentrations to maintain their glycoprotein cell wall … Likewise, is halobacterium Salinarum an Autotroph or Heterotroph? Common Name- N/A. 32) What is the specific action of bacteriorhodopsin? What does halobacterium Salinarum look like? Describe organism- Pill shaped . Family- Halobacteriaceae. Biology 150 Exam 3. It is classified as an extremophile due to its ability to survive in environments with very high salt concentrations. Secondly, is halobacterium Gram positive or negative? Organism- Halobacterium salinarum. The halophilic Archaea (order Halobacteriales) form a diverse group of microorganisms adapted to life at high salt concentrations. The proton gradient which is formed can then be used to generate chemical energy by ATP synthase. Purpose This study utilized the principle that the bacteriorhodopsin (BR) produced by Halobacterium salinarum could increase the hydrogen production of Rhodobacter sphaeroides. Similarly, you may ask, how does halobacterium Salinarum obtain energy? How does the Halobacterium salinarum can survive for short periods without oxygen. Halobacterium salinarum is not a bacterium, but is a model organism from the halophilic branch of Archaea (2). YOU MIGHT ALSO LIKE... 64 terms. This procedured is based on bacterial milking. Halobacterium salinarum is an extremely halophilic marine obligate aerobic archaeon.Despite its name, this is not a bacterium, but rather a member of the domain Archaea. Halophiles, such as Halobacterium salinarum, can generate an electrochemical proton gradient across the membrane by respiration and/or the light driven proton pump bacteriorhodopsin (Michel and Oesterhelt, 1980).This organism generates a SMF by an H + /Na + antiporter that expels the sodium ions and thus keeps the cytoplasm relatively free of sodium (Murakami and Konishi, 1988). Halobacterium salinarum R1 is an extremely halophilic archaeon, able to attach to the surface and to form characteristic biofilm structures under physiological conditions. Bacteriorhodopsins isolated from Halobacterium salinarum have been especially studied for their applications in electronics and optics. To obtain more oxygen H. salinarum produce gas vesicles, which allow them to float to the surface where oxygen levels are higher and more light is available. H. Salinarum is an autotroph and uses chemosynthesis. What does halobacterium mean? Expert Answer . what benefit does bacteriorhodopsin confer on halobacterium salinarum. 8,9 Two versions have been described, from Halobacterium salinarum 183 and from Natronobacterium pharaonis, 184 with somewhat different properties. 31) What does Halobacterium salinarum use the protein bacteriorhodopsin for?  Effect of nitrogen source on growth and bR production. salinarum is a rod-shaped, single-celled, motile microorganism that can live with only light as an energy source due to its retinal protein … Despite its name, this is not a bacterium, but rather a member of the domain Archaea. (A) Normalized cell density of Halobacterium salinarum as a function of time for different salt (NaCl) concentrations. Either a foreign object takes over cells and … Halobacterium salinarum needs high salt concentrations in order to survive. Industrial process for mass production of ectoine and hydroxyectoine were developed by using Halomonas elongata and Marinococcus M52, respectively. Halobacterium salinarum is an extreme halophile archaeon with an absolute requirement for a multimolar salt environment. Thermophilic bacteria are common in soil and volcanic environments i.e. NRC-1 is a non-pathogenic, halophilic archaea that thrives all over the world in high salt environments, including salt production facilities, brine inclusions in salt crystals, natural lakes and ponds, and salt marshes.Prior to 1990 H. NRC-1 was classified as an archeabacterium under the prokaryote kingdom in the two-empire system which consisted of eukaryotes and … Halobacterium salinarum is a model organism for the halophilic branch of the archaea. Halobacterium salinarum is an extremely halophilic marine obligate aerobic archaeon. 2001). It is found in salted fish, hides, hypersaline lakes, and salterns.As these salterns reach the minimum salinity limits for extreme halophiles, their waters become purple or reddish color due to the high densities of … Halobacterium salinarum is a model organism for the halophilic branch of the archaea. The type strain 91-R6 (DSM 3754 T) has seldom been studied and its genome sequence has only recently been determined by our group.The exact relationship between the type strain and … • Halobacterium salinarum • Halobacterium sp. 2. no fermentation, so no new NAD+ for glycolysis 3. uses purple membranes (membranes filled with Bacteriorhodopsin) 4. make energy via light-mediated ATP synthesis a. uses Bacteriorhodopsin (protein) b. uses retinal (carotenoid-like) When Halobacterium salinarum … c) moving water into the cell. They have an aerobic metabolism and can be red or purple. Information and translations of halobacterium in the most comprehensive dictionary definitions resource on the web. Although numerous uses of this protein have been presented, there are yet to be any high-scale commercial … Halobacterium cutirubrum (Lochhead 1934) Elazari-Volcani 1957. Halophilic Archaea (Haloarchaea) thrive in salterns containing sodium chloride concentrations up to saturation. Here, we report on the first insights into H. salinarum biofilm formation when exposed to copper, nickel … Phylum- Euryarchaeota. Dynamic response in live Hbt. MCB 2: Exam 1. Meaning of halobacterium. What does Halobacterium salinarum use the protein bacteriorhodopsin for a from MCB 2610 at University Of Connecticut The glycoprotein has a high negative charge. Presumably, its function is … Agitation is important to circulate air and also helps Halo grow faster. The cells contain molar concentrations of K + and Cl − to provide osmotic balance. Its name is Methanobrevibacter smithii. Enzymes and other proteins require the presence of high salt concentrations for activity and structural stability. Also know, is Halobacteria dangerous? Genome Halobacterium has a genome size of about 2.5 Gbp (Gbp = giga-base-pairs or 10^9 bp). Methods In this … What kingdom does halobacterium belong to? Halobacterium salinarum is an extremely halophilic marine obligate aerobic archaeon. Definition of halobacterium in the Definitions.net dictionary. Uses HtrI- to phototax toward red light and away from UV light Uses HtrII to repell from blue light Both bind sensory rhodopsin, using CheA as sensor kinase and CheY as response regulator. How does Halobacterium salinarum avoid bad light and find good light? Previous question Next question Transcribed … Archaea-> Euryarchaeota-> Halobacteria-> Halobacteriales-> Halobacteriaceae-> Halobacterium-> Halobacterium salinarum-Optimal pH 37 Optimal Temerature -Pathogenicity - Antimicrobial Susceptibility - Spore Forming - Biofilm Forming Extremophile Extreme Environment Negative Gram Stain No Microbiome Location No Plant … Genus- Halobacterium. Thermophiles are thought to be one of the original organisms to have survived on earth over 3 billion years ago, in an environment with much higher … It is found in … … A warm place around 37 degrees Celsius is ideal but not required; the heat simulates a warm climate which causes H. salinarium to grow at a faster rate. 79 terms. This is not surprising, since halobacteria live in extreme environments distinguished by hypersalinity, high solar radiation, and high temperature (Kennedy et al. It is rod-shaped, motile, lives in highly saline environments (4M salt and higher), and is one of the few species known that can live in saturated salt solutions. 1. where does it get energy? Halobacterium salinarum is a rod-shaped, motile bacterium that lives in highly saline environments (4M salt and higher), and is one of the few species known that can live in saturated salt solutions B view the full answer. Halobacterium salinarum. 59 terms. Rather, it uses a single protein, bacteriorhodopsin, in which light energy is absorbed by … It is rod-shaped, motile, lives in highly saline environments (4M salt and higher), and is one of the few species known that can live in saturated salt solutions. a) energy production. Halobacterium salinarum is a model organism for the halophilic branch of the archaea. Halorhodopsin, located in the cell membranes of halobacteria, is a bacteriorhodopsin-like retinal protein but an inward-directed electrogenic chloride ion pump instead of an outward-directed proton pump. Unicellular or multicellular- Unicellular . Cells grown in 4.2 … They possess an S-layered cell wall whose constituent is a high molecular mass glycoprotein. salinarum to hyposaline stress. It is … Chemotaxis is how halobacterium salinarum moves. Many Haloarchaea possess genes encoding gas vesicles, but only a few species, such as Halobacterium salinarum and Haloferax mediterranei, produce these gas-filled, proteinaceous nanocompartments. Despite its name, this is not a bacterium, but rather a member of the domain Archaea. Abstract. She's been studying this single-celled troublemaker and its role in the microbiome for over 5 years. There is a main chromosome and a large plasmid.The large plasmid encodes gas vacuoles and the pigment bacteriorhodopsin. It was discovered 80 years ago when isolated from salted fish, long before the proposal for a third domain was put forward in 1978. NRC-1 or strain ATCC 700922 is adapted to grow under extreme high salinity conditions. d) degradation of large polysaccharides. Two proteins form a novel subfamily and are named ‘SMC‐like proteins of H. salinarum’ (Sph1 and Sph2).Northern blot analyses revealed that sph1 and hp24, the adjacent gene, are solely transcribed in exponentially growing, but not in stationary … How does the organism get food- It does not eat. There are many different transposable sequences (transposons), up to some 5000 bp in length, and each present as 20 or more copies. The Halobacterium salinarum strain VKPM b-11850 superior in productivity (biomass and bacteriorhodopsin) prototype VKPM … Halobacterium sp. ";s:7:"keyword";s:39:"where does halobacterium salinarum live";s:5:"links";s:901:"<a href="https://api.duassis.com/storage/xwqtu/secrets-royal-beach-punta-cana-junior-suite-pool-view">Secrets Royal Beach Punta Cana Junior Suite Pool View</a>,
<a href="https://api.duassis.com/storage/xwqtu/blot-out-crossword-clue-10-letters">Blot Out Crossword Clue 10 Letters</a>,
<a href="https://api.duassis.com/storage/xwqtu/griots-interior-cleaner-vs-meguiars">Griots Interior Cleaner Vs Meguiars</a>,
<a href="https://api.duassis.com/storage/xwqtu/how-to-get-strawberry-blonde-hair-with-overtone">How To Get Strawberry Blonde Hair With Overtone</a>,
<a href="https://api.duassis.com/storage/xwqtu/best-golf-courses-in-punta-cana">Best Golf Courses In Punta Cana</a>,
<a href="https://api.duassis.com/storage/xwqtu/2016-f1-technical-regulations">2016 F1 Technical Regulations</a>,
<a href="https://api.duassis.com/storage/xwqtu/evelop-airlines-flight-status">Evelop Airlines Flight Status</a>,
";s:7:"expired";i:-1;}

Zerion Mini Shell 1.0